cIAP2 Polyklonaler Antikörper

cIAP2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 24304-1-AP

Synonyme

AIP1, API2, Apoptosis inhibitor 2, BIRC3, C IAP2, CIAP2, HAIP1, hIAP 1, HIAP1, IAP 1, IAP homolog C, IAP1, MALT2, MIHC, RING finger protein 49, RNF49

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Galerie der Validierungsdaten

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  WB analysis using 24304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 24304-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 24304-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24304-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Daudi using 24304-1-AP

  IP Result of anti-cIAP2 (IP:24304-1-AP, 4ug; Detection:24304-1-AP 1:1000) with Daudi cells lysate 2800ug.

• 

  IHC staining of human tonsillitis using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24304-1-AP (cIAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24304-1-AP (cIAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, HeLa-Zellen, Raji-Zellen, RAW 264.7-Zellen, U-937-Zellen
• Erfolgreiche IP: Daudi-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Hodengewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 5 publications below

Produktinformation

24304-1-AP bindet in WB, IP, IHC, ELISA cIAP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: cIAP2 fusion protein Ag20143
• Vollständiger Name: baculoviral IAP repeat-containing 3
• Berechnetes Molekulargewicht: 604 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC027485
• Gene symbol: BIRC3
• Gene ID (NCBI): 330
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Dev Cell; Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.; Authors - Maria Zachari
human	WB	Oncotarget; The putative tumor activator ARHGEF3 promotes nasopharyngeal carcinoma cell pathogenesis by inhibiting cellular apoptosis.; Authors - Tian-Hao Liu
human	IHC	Front Oncol; Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.; Authors - Jinguo Zhang
human,mouse	WB	Biomed Pharmacother; Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.; Authors - Lei Wang
human	IHC	Cancer Med; Targeting inhibitors of apoptosis proteins suppresses medulloblastoma cell proliferation via G2/M phase arrest and attenuated neddylation of p21.; Authors - Shu-Mei Chen
human	WB	Cancer Biol Ther; Survivin knockdown combined with apoptin overexpression inhibits cell growth significantly.; Authors - Liu Qin Q