Bim Polyklonaler Antikörper

Bim Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 22037-1-AP

Synonyme

BAM, BCL2L11, BIM, BIM alpha6, BIM beta6, BIM beta7, BimEL, BimL, BOD

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Galerie der Validierungsdaten

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  WB analysis using 22037-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22037-1-AP (BIM, BCL2L11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 22037-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22037-1-AP (BIM, BCL2L11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22037-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22037-1-AP (BIM, BCL2L11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 22037-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22037-1-AP (BIM, BCL2L11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22037-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22037-1-AP (BIM, BCL2L11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 23 publications below
• IHC: See 3 publications below

Produktinformation

22037-1-AP bindet in WB, IHC, ELISA Bim und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Bim fusion protein Ag11875
• Vollständiger Name: BCL2-like 11 (apoptosis facilitator)
• Berechnetes Molekulargewicht: 198 aa, 22 kDa
• Beobachtetes Molekulargewicht: 23 and 18 kDa
• GenBank-Zugangsnummer: BC033694
• Gene symbol: BCL2L11
• Gene ID (NCBI): 10018
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Aging (Albany NY); Extracorporeal shockwave relieves endothelial injury and dysfunction in steroid-induced osteonecrosis of the femoral head via miR-135b targeting FOXO1: in vitro and in vivo studies.; Authors - Xinjie Wu
human	WB	Virulence; Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.; Authors - Wei Jie
human	WB	Oxid Med Cell Longev; One-Two Punch Therapy for the Treatment of T-Cell Malignancies Involving p53-Dependent Cellular Senescence; Authors - Yingjie Qing
mouse	WB	Biochem Pharmacol; Inhibition of IRE1 Suppresses the Catabolic Effect of IL-1β on Nucleus Pulposus Cell and Prevents Intervertebral Disc Degeneration in vivo.; Authors - Honglei Kang
mouse	WB	Mol Cells; Sertad1 Induces Neurological Injury after Ischemic Stroke via the CDK4/p-Rb Pathway.; Authors - Jianxiong Li