BHMT Polyklonaler Antikörper

BHMT Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

31

Rezensionen (1)

Kat-Nr. : 15965-1-AP

Synonyme

BHMT

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse kidney using 15965-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15965-1-AP (BHMT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 15965-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15965-1-AP (BHMT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 15965-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15965-1-AP (BHMT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 15965-1-AP

  IP Result of anti-BHMT (IP:15965-1-AP, 4ug; Detection:15965-1-AP 1:1000) with mouse kidney tissue lysate 4400ug.

• 

  IHC staining of human spleen using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 15965-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15965-1-AP (BHMT antibody) at dilution of 1:100 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, humanes Nierengewebe, L02-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Lungengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 31 publications below
• IHC: See 1 publications below

Produktinformation

15965-1-AP bindet in WB, IP, IHC, ELISA BHMT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BHMT fusion protein Ag8738
• Vollständiger Name: betaine-homocysteine methyltransferase
• Berechnetes Molekulargewicht: 406 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC012616
• Gene symbol: BHMT
• Gene ID (NCBI): 635
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
mouse	WB	NPJ Sci Food; Ablation of the gut microbiota alleviates high-methionine diet-induced hyperhomocysteinemia and glucose intolerance in mice; Authors - Wenqiang Li
	WB	J Anim Sci Biotechnol; Dietary betaine activates hepaticVTGIIexpression in laying hens associated with hypomethylation ofGRgene promoter and enhancedGRexpression.; Authors - Nagmeldin A Omer
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang
pig	WB	J Agric Food Chem; Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.; Authors - Yimin Jia
pig	WB	Sci Rep; Abnormally activated one-carbon metabolic pathway is associated with mtDNA hypermethylation and mitochondrial malfunction in the oocytes of polycystic gilt ovaries.; Authors - Longfei Jia

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Fatemeh (Verified Customer) (05-22-2023)

50 ug of protein was used for WB. Blot was blocked with milk and incubated with 1-1000 primary antibody overnight at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1-1000
• Cell Tissue Type: ESC

BHMT Antibody Western Blot validation (1-1000 dilution) in ESC (Cat no:15965-1-AP)