BDH1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BDH1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 15417-1-AP

Synonyme

BDH, BDH1, SDR9C1

---

Galerie der Validierungsdaten

• 

  WB analysis using 15417-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15417-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15417-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15417-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 15417-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15417-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 15417-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15417-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15417-1-AP

  IP Result of anti-BDH1 (IP:15417-1-AP, 4ug; Detection:15417-1-AP 1:300) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of rat brain using 15417-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15417-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15417-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15417-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15417-1-AP (BDH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COLO 320-Zellen, HT-29-Zellen, humanes Lebergewebe, Mausherzgewebe, Maushirngewebe, Mauslebergewebe, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

15417-1-AP bindet in WB, IP, IHC, IF, ELISA BDH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BDH1 fusion protein Ag7705
• Vollständiger Name: 3-hydroxybutyrate dehydrogenase, type 1
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC005844
• Gene symbol: BDH1
• Gene ID (NCBI): 622
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.; Authors - Patrycja Puchalska
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
mouse	WB	Sci Adv; Ketogenic diet uncovers differential metabolic plasticity of brain cells; Authors - Tim Düking
human	WB	Proc Natl Acad Sci U S A; PMID: 29311302; Authors - Marcus D Goncalves
human,mouse	WB	EMBO Mol Med; Ketone body oxidation increases cardiac endothelial cell proliferation.; Authors - Eva-Maria Weis
mouse	WB	J Cell Biol; CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.; Authors - Désirée Schatton