BCO2 Polyklonaler Antikörper

BCO2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14324-1-AP

Synonyme

B DIOX II, BCO2, Beta carotene dioxygenase 2, beta carotene oxygenase 2

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Galerie der Validierungsdaten

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  WB analysis using 14324-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14324-1-AP (BCO2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 14324-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14324-1-AP (BCO2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14324-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14324-1-AP (BCO2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate hyperplasia using 14324-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14324-1-AP (BCO2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 14324-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14324-1-AP (BCO2 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, L02-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Prostatahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

14324-1-AP bindet in WB, IHC, ELISA BCO2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCO2 fusion protein Ag5590
• Vollständiger Name: beta-carotene oxygenase 2
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC041656
• Gene symbol: BCO2
• Gene ID (NCBI): 83875
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta,beta-carotene 9',10'-oxygenase (BCO2) catalyzes the asymmetric oxidative cleavage of beta-carotene in carotene metabolism.The apocarotenals formed by this enzyme may be the precursors for the biosynthesis of retinoic acid or exert unknown physiological effects. BCDO2 plays an important role in protecting against carotenoid-induced mitochondrial dysfunction and has a broader substrate specificity than previously recognized (PMID:22147584). This antibody detects the N-terminal of BCO2.

Publikationen

Species	Application	Title
human, mouse	WB,IHC	Proc Natl Acad Sci U S A; Inactivity of human β,β-carotene-9',10'-dioxygenase (BCO2) underlies retinal accumulation of the human macular carotenoid pigment.; Authors - Binxing Li
mouse	WB	J Nutr Biochem; Ablation of β,β-carotene-9',10'-oxygenase 2 remodels the hypothalamic metabolome leading to metabolic disorders in mice.; Authors - Xin Guo
mouse	WB	Mol Nutr Food Res; Dietary wolfberry upregulates carotenoid metabolic genes and enhances mitochondrial biogenesis in the retina of db/db diabetic mice.; Authors - Yu Huifeng H
mouse	WB	Mol Nutr Food Res; Wolfberries potentiate mitophagy and enhance mitochondrial biogenesis leading to prevention of hepatic steatosis in obese mice: The role of AMP-activated protein kinase α2 subunit.; Authors - Dingbo Lin
mouse		Molecules; Carotenoid Lutein Selectively Inhibits Breast Cancer Cell Growth and Potentiates the Effect of Chemotherapeutic Agents through ROS-Mediated Mechanisms.; Authors - Xiaoming Gong