BCL2 Monoklonaler Antikörper

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• KD/KO Validated

BCL2 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

4H8C6

Kat-Nr. : CL555-60178

Synonyme

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, Oncogene B cell leukemia 2

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Galerie der Validierungsdaten

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  IF Staining of human appendicitis using CL555-60178

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  IF Staining of human appendicitis using CL555-60178

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  IF Staining of NIH/3T3 using CL555-60178

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  IF Staining of MCF-7 using CL555-60178

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  FC experiment of HeLa using CL555-60178

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human BCL2 (CL555-60178, Clone:4H8C6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Appendizitis-Gewebe, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-60178 bindet in IF, FC (Intra) BCL2 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BCL2 fusion protein Ag3508
• Vollständiger Name: B-cell CLL/lymphoma 2
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC027258
• Gene symbol: BCL2
• Gene ID (NCBI): 596
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

1) What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.