BCL2 Monoklonaler Antikörper

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• KD/KO Validated

BCL2 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4H8C6

Kat-Nr. : CL488-60178

Synonyme

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, Oncogene B cell leukemia 2

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Galerie der Validierungsdaten

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  IF Staining of human tonsillitis using CL488-60178

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488-conjugated BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:100.

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  IF Staining of human tonsillitis using CL488-60178

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488-conjugated BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:100.

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  IF Staining of human appendicitis using CL488-60178

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 488 BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  IF Staining of human appendicitis using CL488-60178

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 488 BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  IF Staining of MCF-7 using CL488-60178

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  FC experiment of Jurkat using CL488-60178

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human BCL2 (CL488-60178, Clone:4H8C6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe, humanes Appendizitis-Gewebe, MCF-7-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-60178 bindet in IF, FC (Intra) BCL2 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BCL2 fusion protein Ag3508
• Vollständiger Name: B-cell CLL/lymphoma 2
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC027258
• Gene symbol: BCL2
• Gene ID (NCBI): 596
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).