BCL10 Polyklonaler Antikörper

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• KD/KO Validated

BCL10 Polyklonal Antikörper für FC, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 50180-1-AP

Synonyme

B cell CLL/lymphoma 10, B cell lymphoma/leukemia 10, BCL10, c E10, CARD like apoptotic protein, CARMEN, cCARMEN, Cellular E10, Cellular homolog of vCARMEN, CIPER, CLAP, hCLAP, mE10

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 50180-1-AP

  WB result of BCL10 antibody (50180-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL10 transfected K-562 cells.

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  WB analysis using 50180-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 50180-1-AP (BCL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 50180-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 50180-1-AP (BCL10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 50180-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 50180-1-AP (BCL10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 50180-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 50180-1-AP (BCL10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 50180-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 50180-1-AP (BCL10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Jurkat using 50180-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human BCL10 (50180-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Jurkat-Zellen, K-562-Zellen, Maus-Eierstockgewebe, Raji-Zellen, Ramos-Zellen, Ratten-Eierstockgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

50180-1-AP bindet in WB, IHC, FC, ELISA BCL10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCL10 fusion protein Ag0037
• Vollständiger Name: B-cell CLL/lymphoma 10
• Berechnetes Molekulargewicht: 233 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26-32 kDa
• GenBank-Zugangsnummer: BC053617
• Gene symbol: BCL10
• Gene ID (NCBI): 8915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The gene encoding BCL10 was identified by its recurrent translocation t(1;14)(p22;q32) in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. BCL10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB.

Publikationen

Species	Application	Title
mouse	WB	J Biol Chem; The IRAK-1-BCL10-MALT1-TRAF6-TAK1 cascade mediates signaling to NF-kappaB from Toll-like receptor 4.; Authors - Dong Wei W
mouse	WB	J Biol Chem; BCL10 mediates lipopolysaccharide/toll-like receptor-4 signaling through interaction with Pellino2.; Authors - Liu Yingle Y ; KD Validated
human	WB	Cell Signal; The paracaspase MALT1 is a downstream target of Smad3 and potentiates the crosstalk between TGF-β and NF-kB signaling pathways in cancer cells; Authors - Fatma Aybuke Mazi