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  • KD/KO Validated

BCCIP Polyklonaler Antikörper

BCCIP Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16043-1-AP

Synonyme

BCCIP, Protein TOK 1, TOK 1, TOK1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, A549-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IFHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16043-1-AP bindet in WB, IP, IHC, IF, ELISA BCCIP und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BCCIP fusion protein Ag8934
Vollständiger Name BRCA2 and CDKN1A interacting protein
Berechnetes Molekulargewicht 314 aa, 36 kDa
Beobachtetes Molekulargewicht 50-58 kDa
GenBank-ZugangsnummerBC009771
Gene symbol BCCIP
Gene ID (NCBI) 56647
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCCIP (or TOK1) is isolated as a BRCA2- and CDKN1A (p21)-interacting protein and is implicated in homologous recombination (HR) pathway and inhibition of DNA replication stress. BCCIP is an evolutionarily conserved nuclear protein with multiple interacting domains. BCCIP deficiency in mice impaired embryonic and postnatal neural development, causing severe ataxia, cerebral and cerebellar defects, and microcephaly, which are associated with spontaneous DNA damage and subsequent cell death in the proliferative cell populations of the neural system during embryogenesis. BCCIP is essential for maintaining the transactivation activity of wild type p53 suggesting a potential role of BCCIP in cancer etiology.

Protokolle

Produktspezifische Protokolle
WB protocol for BCCIP antibody 16043-1-APProtokoll herunterladen
IF protocol for BCCIP antibody 16043-1-APProtokoll herunterladen
IP protocol for BCCIP antibody 16043-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Int J Mol Sci

The Males Absent on the First (MOF) Mediated Acetylation Alters the Protein Stability and Transcriptional Activity of YY1 in HCT116 Cells

Authors - Tingting Wu
humanWB

Int J Mol Sci

Feedback Modulation between Human INO80 Chromatin Remodeling Complex and miR-372 in HCT116 Cells

Authors - Junaid Ali Shah
humanWB,IF

FEBS J

BCCIP binds to and activates its promoter in a YY1-dependent fashion in HCT116 cells.

Authors - Yi Sui
  • KD Validated
humanWB,IF

Protein Cell

Human INO80/YY1 chromatin remodeling complex transcriptionally regulates the BRCA2- and CDKN1A-interacting protein (BCCIP) in cells.

Authors - Jiaming Su
humanWB

Biochem Biophys Res Commun

BCCIPβ facilitates p53 ubiquitination via binding with E6 protein in high-risk HPV positive head and neck squamous cell carcinoma.

Authors - Fang Wang
humanWB,IHC

Int J Oncol

Differential BCCIP gene expression in primary human ovarian cancer, renal cell carcinoma and colorectal cancer tissues.

Authors - Liu Xiaoxia X