BCAS2 Monoklonaler Antikörper

BCAS2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A12G1

Kat-Nr. : 68003-1-Ig

Synonyme

BCAS2, DAM1, Pre mRNA splicing factor SPF27

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Galerie der Validierungsdaten

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  WB analysis using 68003-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis of LNCaP using 68003-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68003-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 68003-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68003-1-Ig bindet in WB, ELISA BCAS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BCAS2 fusion protein Ag21309
• Vollständiger Name: breast carcinoma amplified sequence 2
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC005285
• Gene symbol: BCAS2
• Gene ID (NCBI): 10286
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Breast carcinoma amplified sequence 2 (BCAS2) is preferentially known as pre-mRNA splicing factor SPF27 and was originally characterized as an up-regulated gene by amplification in human breast cancer cells. BCAS2 has been shown to be involved in DNA damage repair through the replication protein A (RPA) complex.