BBX Polyklonaler Antikörper

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• KD/KO Validated

BBX Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 17254-1-AP

Synonyme

BBX, Bobby sox homolog, bobby sox homolog (Drosophila), HBP2, HMG box containing protein 2, HSPC339, MDS001

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 17254-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17254-1-AP (BBX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17254-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17254-1-AP (BBX antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17254-1-AP

  IP Result of anti-BBX (IP:17254-1-AP, 4ug; Detection:17254-1-AP 1:1000) with HepG2 cells lysate 2400ug.

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  IHC staining of human ovary using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17254-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe, humanes Hirngewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

17254-1-AP bindet in WB, IP, IHC, IF, ELISA BBX und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BBX fusion protein Ag10691
• Vollständiger Name: bobby sox homolog (Drosophila)
• Berechnetes Molekulargewicht: 941 aa, 105 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC012837
• Gene symbol: BBX
• Gene ID (NCBI): 56987
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BBX, also named HBP2, is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As a DNA-binding domain in HBP2, HMG is essential for its function in Schizosaccharomyces pombe. HBP2 activates CdcIO-dependent transcription at G1/s and allows growth of cdc10-129, resldelta and rep2delta mutant cells at the restrictive temperature

Publikationen

Species	Application	Title
rat	WB,IHC,IF	Biol Reprod; PiggyBac Transposon-Mediated Mutagenesis in Rats Reveals a Crucial Role of Bbx in Growth and Male Fertility.; Authors - Chieh-Ying Wang ; KO Validated
zebrafish	IHC	Dev Genes Evol; Characterization of Bbx, a member of a novel subfamily of the HMG-box superfamily together with Cic.; Authors - TieLin Chen
	WB	Dev Comp Immunol; Discovery of BbX transcription factor in the patagonian blennie: Exploring expression changes following combined bacterial and thermal stress exposure; Authors - Danixa Martínez