BAG3 Monoklonaler Antikörper

BAG3 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

4G6C2

Kat-Nr. : 68076-1-Ig

Synonyme

BAG 3, BAG3, BIS, CAIR 1, Docking protein CAIR 1

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Galerie der Validierungsdaten

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  WB analysis using 68076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 68076-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using BAG3 antibody (68076-1-Ig, Clone: 4G6C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HeLa-Zellen, Kaninchenherzgewebe, LNCaP-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

68076-1-Ig bindet in WB, IHC, IF, ELISA BAG3 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BAG3 fusion protein Ag17608
• Vollständiger Name: BCL2-associated athanogene 3
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC006418
• Gene symbol: BAG3
• Gene ID (NCBI): 9531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy.

Publikationen

Species	Application	Title
human,rat	IHC	J Physiol Biochem; BAG3 protects chondrocytes against lumbar facet joint osteoarthritis by regulating autophagy and apoptosis.; Authors - Xin Lu
rat	WB,IF	J Physiol Sci; High-intensity interval training alleviates exhaustive exercise-induced HSP70-assisted selective autophagy in skeletal muscle; Authors - Jiao Lu