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  • Featured Product
  • KD/KO Validated

BAG1 Polyklonaler Antikörper

BAG1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19064-1-AP

Synonyme

BAG 1, BAG1, BAG1L speicfic, BAG1M, BAG1S, HAP, RAP46

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HeLa cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HeLa using 19064-1-AP

    WB result of BAG1 antibody (19064-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG1 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HeLa using 19064-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of UV treated Hela using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of UV treated Hela using 19064-1-AP

    UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HL-60 using 19064-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of MCF-7 using 19064-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of Jurkat using 19064-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of MCF-7 using 19064-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HEK-293 using 19064-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of RAW264.7 using 19064-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HT-1080 using 19064-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using BAG1 Polyclonal antibody (19064-1-AP)

    IP experiment of HeLa using 19064-1-AP

    IP Result of anti-BAG1 (IP:19064-1-AP, 3ug; Detection:19064-1-AP 1:500) with HeLa cells lysate 3440ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using BAG1 Polyclonal antibody (19064-1-AP)

    IHC staining of human testis using 19064-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using BAG1 Polyclonal antibody (19064-1-AP)

    IHC staining of human testis using 19064-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using BAG1 Polyclonal antibody (19064-1-AP)

    IHC staining of human ovary using 19064-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using BAG1 Polyclonal antibody (19064-1-AP)

    IHC staining of human ovary using 19064-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BAG1 Polyclonal antibody (19064-1-AP)

    IF Staining of HeLa using 19064-1-AP

    Immunofluorescent analysis of HeLa cells, using BAG1 antibody 19064-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BAG1 Polyclonal antibody (19064-1-AP)

    IF Staining of MCF-7 using 19064-1-AP

    Immunofluorescent analysis of MCF-7 cells, using BAG1 antibody 19064-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HEK-293-Zellen, HL-60-Zellen, HT-1080.Zellen, Jurkat-Zellen, MCF-7-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Hodengewebe, humanes Eierstockgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

19064-1-AP bindet in WB, IP, IHC, IF, ELISA BAG1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BAG1 fusion protein Ag13555
Vollständiger Name BCL2-associated athanogene
Berechnetes Molekulargewicht 39 kDa
Beobachtetes Molekulargewicht 50-55 kDa, 30-33 kDa
GenBank-ZugangsnummerBC014774
Gene symbol BAG1
Gene ID (NCBI) 573
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody can recognize all the isoforms of BAG1.

Protokolle

Produktspezifische Protokolle
WB protocol for BAG1 antibody 19064-1-APProtokoll herunterladen
IHC protocol for BAG1 antibody 19064-1-APProtokoll herunterladen
IF protocol for BAG1 antibody 19064-1-APProtokoll herunterladen
IP protocol for BAG1 antibody 19064-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle

Oncol Rep

Magnetic gold nanoparticle-mediated small interference RNA silencing Bag-1 gene for colon cancer therapy.

Authors - Wenbai Huang
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tongbin (Verified Customer) (08-25-2020)

This BAG1 antibody works very well in detecting endogenous BAG1 protein by western blots.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart