B4GALT6 Polyklonaler Antikörper

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• KD/KO Validated

B4GALT6 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20148-1-AP

Synonyme

B4Gal T6, B4GALT6, Beta 1,4 GalTase 6, beta4Gal T6, LacCer synthase

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 20148-1-AP

  WB result of B4GALT6 antibody (20148-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B4GALT6 transfected HEK-293 cells.

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  WB analysis of mouse heart using 20148-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20148-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20148-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20148-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 20148-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20148-1-AP (B4GALT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 20148-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20148-1-AP (B4GALT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 20148-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20148-1-AP (B4GALT6 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 20148-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20148-1-AP (B4GALT6 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 20148-1-AP

  Immunofluorescent analysis of Hela cells, using B4GALT6 antibody 20148-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HEK-293-Zellen, Maushirngewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

20148-1-AP bindet in WB, IHC, IF, ELISA B4GALT6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: B4GALT6 fusion protein Ag13686
• Vollständiger Name: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6
• Berechnetes Molekulargewicht: 382 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45-60 kDa
• GenBank-Zugangsnummer: BC074884
• Gene symbol: B4GALT6
• Gene ID (NCBI): 9331
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta-1,4-galactosyltransferase 6 (B4GALT6) is a member of the beta 4-galactosyltransferase family that catalyzes the biosynthesis of glycosphingolipids in UDP-dependent manner (PMID: 23744354). B4GALT6 in astrocytes boosts CNS inflammation and promote neurodegeneration during chronic CNS inflammation (PMID: 25216636).

Publikationen

Species	Application	Title
mouse	IF	Nat Med; Regulation of astrocyte activation by glycolipids drives chronic CNS inflammation.; Authors - Lior Mayo
human	IHC	Biochim Biophys Acta Mol Cell Biol Lipids; Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors.; Authors - Jiřina Procházková