Arp5/ACTR5 Monoklonaler Antikörper

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• KD/KO Validated

Arp5/ACTR5 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E11C1

Kat-Nr. : 67326-1-Ig

Synonyme

Actin related protein 5, ACTR5, Arp5, Arp5/ACTR5, FLJ12785, hARP5, INO80M, Sarcoma antigen NY SAR 16

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Galerie der Validierungsdaten

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  WB analysis using 67326-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67326-1-Ig

  WB result of Arp5/ACTR5 antibody (67326-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arp5/ACTR5 transfected HEK-293 cells.

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  WB analysis of C2C12 using 67326-1-Ig

  C2C12 cells were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig skeletal muscle using 67326-1-Ig

  pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 67326-1-Ig

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, C2C12-Zellen, Hausschwein-Skelettmuskelgewebe, HEK-293-Zellen, K-562-Zellen, Ratten-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67326-1-Ig bindet in WB, ELISA Arp5/ACTR5 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Arp5/ACTR5 fusion protein Ag15976
• Vollständiger Name: ARP5 actin-related protein 5 homolog (yeast)
• Berechnetes Molekulargewicht: 607 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC038402
• Gene symbol: ACTR5
• Gene ID (NCBI): 79913
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)