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Apolipoprotein AI Polyklonaler Antikörper

Apolipoprotein AI Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-14427

Synonyme

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CoraLite®594-conjugated Apolipoprotein AI Polyclon (CL594-14427)

    IF Staining of mouse liver using CL594-14427

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite®594 Apolipoprotein AI antibody (CL594-14427) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated Apolipoprotein AI Polyclon (CL594-14427)

    FC experiment of HepG2 using CL594-14427

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Apolipoprotein AI (CL594-14427) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFMauslebergewebe
Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-14427 bindet in IF, FC (Intra) Apolipoprotein AI und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Apolipoprotein AI fusion protein Ag5793
Vollständiger Name apolipoprotein A-I
Berechnetes Molekulargewicht 31 kDa
Beobachtetes Molekulargewicht 26-30 kDa
GenBank-ZugangsnummerBC005380
Gene symbol APOA1
Gene ID (NCBI) 335
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 Apolipoprotein AI antibody CL594-14427Protokoll herunterladen
FC protocol for CL594 Apolipoprotein AI antibody CL594-14427Protokoll herunterladen
Standard-Protokolle
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