Angiopoietin 1 Polyklonaler Antikörper

Angiopoietin 1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 27093-1-AP

Synonyme

AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, ANGPT1, KIAA0003

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Galerie der Validierungsdaten

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  WB analysis using 27093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of U-87 MG using 27093-1-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, HeLa-Zellen, Mausherzgewebe, Mauslungengewebe, Rattenlungengewebe, U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

27093-1-AP bindet in WB, ELISA Angiopoietin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Angiopoietin 1 fusion protein Ag25786
• Vollständiger Name: angiopoietin 1
• Berechnetes Molekulargewicht: 498 aa, 58 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC152411
• Gene symbol: ANGPT1
• Gene ID (NCBI): 284
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

Publikationen

Species	Application	Title
human	WB	J Ethnopharmacol; Lycium barbarum polysaccharides attenuates high glucose-induced diabetic retinal angiogenesis by rescuing the expression of miR-15a-5p in RF/6A cells; Authors - Yafei Zhu