Amylin Polyklonaler Antikörper

Amylin Polyklonal Antikörper für IHC,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22305-1-AP

Synonyme

Amylin, DAP, Diabetes associated peptide, IAP, IAPP, islet amyloid polypeptide

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Galerie der Validierungsdaten

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  IHC staining of human pancreas using 22305-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22305-1-AP (Amylin Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 22305-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22305-1-AP (Amylin Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse pancreas using 22305-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 22305-1-AP (Amylin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 22305-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 22305-1-AP (Amylin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 22305-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 22305-1-AP (Amylin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 22305-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 22305-1-AP (Amylin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, Maus-Pankreasgewebe, Ratten-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22305-1-AP bindet in IHC,ELISA Amylin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: islet amyloid polypeptide
• Berechnetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: NM_000415
• Gene symbol: IAPP
• Gene ID (NCBI): 3375
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IAPP, also known as Amylin, is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring a tumor of the pancreas that is derived from beta cells and secretes INS. IAPP is co-secreted with INS by pancreatic β-cells and largely contributes to glycemic control. Studies suggest that this protein, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes.