Alpha Sarcoglycan Monoklonaler Antikörper

Alpha Sarcoglycan Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B10H7

Kat-Nr. : 67078-1-Ig

Synonyme

50 DAG, 50DAG, A2, adhalin, ADL, Alpha sarcoglycan, Alpha SG, DAG2, DMDA2, Dystroglycan 2, LGMD2D, SCARMD1, SGCA

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Galerie der Validierungsdaten

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  WB analysis using 67078-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 67078-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 67078-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67078-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 67078-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 67078-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67078-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67078-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse tongue using 67078-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse tongue using 67078-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 67078-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 67078-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 67078-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 67078-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 67078-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 67078-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 67078-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 67078-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe, Hausschwein-Herzgewebe, Hausschwein-Skelettmuskelgewebe, humanes Herzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Lebergewebe, Mausherzgewebe, Mauszungengewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausherzgewebe, Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67078-1-Ig bindet in WB, IHC, IF, ELISA Alpha Sarcoglycan und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Alpha Sarcoglycan fusion protein Ag28800
• Vollständiger Name: sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
• Berechnetes Molekulargewicht: 387 aa, 43 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC025702
• Gene symbol: SGCA
• Gene ID (NCBI): 6442
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha-sarcoglycan is a member of the sarcoglycan-sarcospan complex (SG-SSPN), which is constituted by α/ɛ-, β-, γ-, and δ-SGs as well as SSPN. Alpha-sarcoglycan gene expression is exclusive of striated muscle and is finely regulated during myogenic differentiation, where the amount of alpha-sarcoglycan transcript is selectively increased after differentiation to myotubes (MTs). Alpha sarcoglycan's disruption causes limb-girdle muscular dystrophy.