Acetyl-Histone H3 (Lys27) Rekombinanter Antikörper

Acetyl-Histone H3 (Lys27) Rekombinant Antikörper für Dot Blot, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Dot Blot, ELISA

Konjugation

Unkonjugiert

CloneNo.

1M16

Kat-Nr. : 82902-1-RR

Synonyme

H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, H3K27ac, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 82902-1-RR

  Various cell lysates were subjected to SDS PAGE followed by western blot with 82902-1-RR (Mono-Acetyl-Histone H3 (Lys27) antibody) at dilution of 1:9800 incubated at room temperature for 1.5 hours.

• 

  Dot Blot experiment of peptide using 82902-1-RR

  Dot blot analysis was used to confirm the specificity of Histone H3K27ac antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (ug/mL) spotted is indicated next to each row. Column 1: H3K27ac. Column 2: Unmodified H3K27. Column 3: H3K9ac. Column 4: H3K14ac. Column 5: H3K18ac. Column 6: H3K23ac. Column 7: H3K36ac. Column 8: H4K5ac. Column 9: H4K8ac. Column 10: H4K12ac. Column 11: H4K16ac. Column 12: H2AK5ac. Column 13: Blank(PBS).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, Mausnierengewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in Dot Blot: peptide

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:19600
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82902-1-RR bindet in WB, Dot Blot, ELISA Acetyl-Histone H3 (Lys27) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes. Acetyl-Histone H3 (Lys27) is enhancer specific mark and plays positive role in gene expression.