AZI2/NAP1 Polyklonaler Antikörper

AZI2/NAP1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15042-1-AP

Synonyme

5 azacytidine induced 2, AZ2, AZI2, AZI2/NAP1, Nak associated protein 1, NAP1, TILP

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Galerie der Validierungsdaten

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  WB analysis using 15042-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15042-1-AP (AZI2/NAP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15042-1-AP

  IP result of anti-AZI2/NAP1(IP:15042-1-AP, 4ug; Detection:15042-1-AP 1:8000) with HEK-293 cells lysate 1400 ug.

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  IP experiment of HepG2 using 15042-1-AP

  IP result of anti-AZI2/NAP1 (IP:15042-1-AP, 4ug; Detection:15042-1-AP 1:300) with HepG2 cells lysate 3000 ug.

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  IHC staining of mouse testis using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15042-1-AP (AZI2/NAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15042-1-AP (AZI2/NAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15042-1-AP (AZI2/NAP1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15042-1-AP (AZI2/NAP1 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15042-1-AP (AZI2/NAP1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15042-1-AP (AZI2/NAP1 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15042-1-AP (AZI2/NAP1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15042-1-AP (AZI2/NAP1 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15042-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AZI2/NAP1 antibody (15042-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15042-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AZI2/NAP1 antibody (15042-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Hodengewebe, humanes Magengewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15042-1-AP bindet in WB, IP, IHC, IF, ELISA AZI2/NAP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AZI2/NAP1 fusion protein Ag6892
• Vollständiger Name: 5-azacytidine induced 2
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC001139
• Gene symbol: AZI2
• Gene ID (NCBI): 64343
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AZI2, also known as NAP1, is a TNF receptor (TNFR)-associated factor family member-associated NF-κB activator-binding kinase 1-binding protein that regulates the production of IFNs. It is an adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity.

Publikationen

Species	Application	Title
human	WB	Cancer Res; FIP200 suppresses immune checkpoint therapy responses in breast cancers by limiting AZI2/TBK1/IRF signaling independent of its canonical autophagy function.; Authors - Takako Okamoto
human	WB	PLoS Pathog; MAVS activates TBK1 and IKKε through TRAFs in NEMO dependent and independent manner.; Authors - Run Fang
human	WB	Front Immunol; Increased IKKϵ protein stability ensures efficient type I interferon responses in conditions of TBK1 deficiency; Authors - Julia Wegner
human	WB,IHC,IF	Autophagy; AZI2 mediates TBK1 activation at unresolved selective autophagy cargo receptor complexes with implications for CD8 T-cell infiltration in breast cancer; Authors - Syn Kok Yeo
human	WB	J Cell Biol; NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis; Authors - Swagatika Paul