AZI1 Polyklonaler Antikörper

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• KD/KO Validated

AZI1 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (1)

Kat-Nr. : 25735-1-AP

Synonyme

5 azacytidine induced 1, AZ1, AZI1, Centrosomal protein of 131 kDa, Cep131, KIAA1118

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 25735-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25735-1-AP (AZI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 25735-1-AP

  Immunofluorescent analysis of HeLa cells using 25735-1-AP (AZI1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

25735-1-AP bindet in WB, IP, IF, ELISA AZI1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AZI1 fusion protein Ag22699
• Vollständiger Name: 5-azacytidine induced 1
• Berechnetes Molekulargewicht: 1083 aa, 122 kDa
• Beobachtetes Molekulargewicht: 131 kDa
• GenBank-Zugangsnummer: BC136578
• Gene symbol: AZI1
• Gene ID (NCBI): 22994
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.

Publikationen

Species	Application	Title
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
human	WB,IF,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li ; KD Validated
human,mouse	IF	Elife; Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis; Authors - Emma A Hall
human	IF	PLoS Biol; Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium.; Authors - Özge Z Aydin
human	WB	Front Oncol; USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.; Authors - Catherine M Nickson ; KD Validated
human	IF	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jay (Verified Customer) (10-15-2019)

Methanol fixation,Immunostaining with Azi1(red), centrosome marker gamma-tubulin(green) and DAPI(blue) for nucleus.First time to show IF result using mouse NIH3T3 cell line.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse NIH3T3

AZI1 Antibody Immunofluorescence, validation (1:100 dilution) in Mouse NIH3T3 (Cat no:25735-1-AP)