Zinc Alpha 2 Glycoprotein Monoklonaler Antikörper

Zinc Alpha 2 Glycoprotein Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

4B8A4

Kat-Nr. : 66178-1-Ig

Synonyme

AZGP1, ZA2G, ZAG, Zinc alpha 2 glycoprotein, Zinc-alpha-2-glycoprotein, Zn alpha 2 glycoprotein, Zn alpha 2 GP, ZNGP1

---

Galerie der Validierungsdaten

• 

  WB analysis of human plasma using 66178-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66178-1-Ig (AZGP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 66178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 66178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 66178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 66178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 66178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human breast cancer using 66178-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Zinc Alpha 2 Glycoprotein antibody (66178-1-Ig, Clone: 4B8A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Prostatahyperplasie-Gewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

66178-1-Ig bindet in WB, IHC, IF, ELISA Zinc Alpha 2 Glycoprotein und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Zinc Alpha 2 Glycoprotein fusion protein Ag5661
• Vollständiger Name: alpha-2-glycoprotein 1, zinc-binding
• Berechnetes Molekulargewicht: 298 aa, 34 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC033830
• Gene symbol: AZGP1
• Gene ID (NCBI): 563
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc-alpha-2-glycoprotein (AZGP1), a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract, and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).

Publikationen

Species	Application	Title
human	WB,IHC	Onco Targets Ther; Reduction of AZGP1 predicts poor prognosis in esophageal squamous cell carcinoma patients in Northern China.; Authors - Hong Tang