ATXN2L Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATXN2L Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 24822-1-AP

Synonyme

A2D, A2LG, A2LP, A2RP, Ataxin 2 domain protein, ataxin 2 like, Ataxin 2 like protein, Ataxin 2 related protein, ATXN2L

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Galerie der Validierungsdaten

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  WB analysis using 24822-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 24822-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24822-1-AP

  IP Result of anti-ATXN2L (IP:24822-1-AP, 4ug; Detection:24822-1-AP 1:2000) with HeLa cells lysate 2400ug.

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  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 24822-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ATXN2L antibody (24822-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, MKN-45-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

24822-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ATXN2L und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATXN2L fusion protein Ag20581
• Vollständiger Name: ataxin 2-like
• Berechnetes Molekulargewicht: 1062 aa, 112 kDa
• Beobachtetes Molekulargewicht: ~140 kDa
• GenBank-Zugangsnummer: BC082760
• Gene symbol: ATXN2L
• Gene ID (NCBI): 11273
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATXN2L is a paralog of Ataxin-2 (ATXN2) but without abnormal polyQ expended track, which is conserved in most of the ATXNs and drives the pathogenesis of neurodegeneration. It was reported that ATXN2L was widely expressed in immortalized cell lines, and ATXN2L-JAK2 fusion was found in CD4-positive T-cell lymphoma.

Publikationen

Species	Application	Title
mouse	IF,WB	Cell; Circadian clocks are modulated by compartmentalized oscillating translation; Authors - Yanrong Zhuang
mouse	WB	Int J Mol Sci; Mid-Gestation lethality of Atxn2l-Ablated Mice.; Authors - Jana Key
human	WB	ACS Chem Biol; Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling.; Authors - Luam E Araya
rat	WB	Phytother Res; Jatrorrhizine ameliorates Schwann cell myelination via inhibiting HDAC3 ability to recruit Atxn2l for regulating the NRG1-ErbB2-PI3K-AKT pathway in diabetic peripheral neuropathy mice; Authors - Xiaobao Gong
human	WB,CoIP	Cell; Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules; Authors - Qinqin Cui
human	WB	Cell Genom; Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders; Authors - Greta Pintacuda

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sahil (Verified Customer) (06-21-2019)

I have checked others too and this works perfectly fine. If needed again would definitely buy it.

• Applications: Western Blot,

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