ATP6V0A4 Polyklonaler Antikörper

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• KD/KO Validated

ATP6V0A4 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 21570-1-AP

Synonyme

A4, ATP6N1B, ATP6N2, ATP6V0A4, RDRTA2, RTA1C, RTADR, STV1, V ATPase 116 kDa isoform a4, VPH1, VPP2

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Galerie der Validierungsdaten

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  WB analysis using 21570-1-AP

  Mouse kidney lysates were subjected to SDS PAGE followed by western blot with 21570-1-AP (ATP6V0A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 21570-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21570-1-AP (ATP6V0A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

21570-1-AP bindet in WB, IHC, ELISA ATP6V0A4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP6V0A4 fusion protein Ag16095
• Vollständiger Name: ATPase, H+ transporting, lysosomal V0 subunit a4
• Berechnetes Molekulargewicht: 840 aa, 96 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC109305
• Gene symbol: ATP6V0A4
• Gene ID (NCBI): 50617
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ATP6V0A4 is a component of vacuolar-H+ATPase (V-ATPase) which is a multi-subunit enzyme that couples ATP hydrolysis to proton pumping across membranes. The V-ATPases are comprised of two major parts, the cytosolic V1 domain involved in ATP-binding and subsequent hydrolysis, and the membrane-associated V0 domain responsible for proton translocation. The V0 domain is composed of five subunits: a, c, c', c'' and d. The 'a' subunit of V0 domain has four isoforms : a1-a4. It has been found that mutations in ATP6V0A4(a4) are associated with distal renal tubular acidosis(dRTA) combined in some cases with progressive hearing loss leading to sensorineural deafness. This antibody was generated against the internal region of human ATP6V0A4 and is predicted to detect the a4 isoform only.

Publikationen

Species	Application	Title
human	IHC	Oncol Lett; Identification of ATP6V0A4 as a potential biomarker in renal cell carcinoma using integrated bioinformatics analysis; Authors - Jinming Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christopher (Verified Customer) (12-14-2020)

We used the anti-ATP6V0A4 antibody. In lysates of homozygous Atp6v0a4-KO mice (described in Hennings et al., EMBO Mol Med, 2012) the signal for ATP6V0A4 was not detected anymore. Thus, the antibody is specific and does not cross-react.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Kidney lysate, IN-IC and HK-2 cells

ATP6V0A4 Antibody Western Blot validation (1:1000 dilution) in Kidney lysate, IN-IC and HK-2 cells (Cat no:21570-1-AP)