ATP5H Polyklonaler Antikörper

ATP5H Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (2)

Kat-Nr. : 17589-1-AP

Synonyme

ATP5H, ATP5JD, ATPase subunit d, ATPQ

---

Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 17589-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17589-1-AP (ATP5H antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 17589-1-AP

  IP Result of anti-ATP5H (IP:17589-1-AP, 3ug; Detection:17589-1-AP 1:2000) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of human lung cancer using 17589-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17589-1-AP (ATP5H antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 17589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17589-1-AP (ATP5H Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 17589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17589-1-AP (ATP5H Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 17589-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17589-1-AP (ATP5H antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Mauslebergewebe, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

17589-1-AP bindet in WB, IP, IHC, IF, ELISA ATP5H und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP5H fusion protein Ag11429
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
• Berechnetes Molekulargewicht: 137 aa, 16 kDa
• Beobachtetes Molekulargewicht: 19-22 kDa
• GenBank-Zugangsnummer: BC032245
• Gene symbol: ATP5H
• Gene ID (NCBI): 10476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.

Publikationen

Species	Application	Title
human	WB	Brain; Ligand-free mitochondria-localized mutant AR-induced cytotoxicity in spinal bulbar muscular atrophy.; Authors - Xia Feng
mouse	WB	Theranostics; AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages.; Authors - Liangjun Xia
human	IHC	Brain Pathol; Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus.; Authors - Margalida A Frau-Méndez
human	WB	Oncol Lett; Dihydroartemisinin inhibits liver cancer cell migration and invasion by reducing ATP synthase production through CaMKK2/NCLX; Authors - Jiang Chang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

SITING (Verified Customer) (05-25-2021)

good

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

ATP5H Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:17589-1-AP)

FH

Zee (Verified Customer) (01-28-2020)

It worked well with western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue extract