ATP5D Polyklonaler Antikörper

ATP5D Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 14893-1-AP

Synonyme

ATP5D, F ATPase delta subunit

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Galerie der Validierungsdaten

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  WB analysis using 14893-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14893-1-AP (ATP5D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14893-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14893-1-AP (ATP5D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14893-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14893-1-AP (ATP5D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 1 publications below

Produktinformation

14893-1-AP bindet in WB, IHC, IF, ELISA ATP5D und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP5D fusion protein Ag6682
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: BC002389
• Gene symbol: ATP5D
• Gene ID (NCBI): 513
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mitochondrial F1Fo-ATP synthase complex uses energy derived from a proton gradient to synthesize ATP. The ATP5D gene encodes the delta subunit of the mitochondrial ATP synthase F1 complex and belongs to the ATPase epsilon chain family. This gene encode the full length protein of 17 kDa with a transit peptide of 22 amino acid.

Publikationen

Species	Application	Title
mouse	WB	Theranostics; AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages.; Authors - Liangjun Xia
human	WB	Int J Mol Sci; CA9 Silencing Promotes Mitochondrial Biogenesis, Increases Putrescine Toxicity and Decreases Cell Motility to Suppress ccRCC Progression.; Authors - Jiatong Xu
mouse	WB	Front Aging Neurosci; Hippocampus-Based Mitochondrial Respiratory Function Decline Is Responsible for Perioperative Neurocognitive Disorders.; Authors - Keqiang He
human	WB	Am J Physiol Cell Physiol; 3-MST/H2S protects cerebral endothelial cells against OGD/R-induced injury via mitoprotection and inhibition of the RhoA/ROCK pathway.; Authors - Fang Zhang
human	IF	J Neuropathol Exp Neurol; Altered Mitochondria, Protein Synthesis Machinery, and Purine Metabolism Are Molecular Contributors to the Pathogenesis of Creutzfeldt-Jakob Disease.; Authors - Belén Ansoleaga
human	WB	Cell Death Dis; Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.; Authors - Anaïs Aulas