ATP1B3 Monoklonaler Antikörper

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• KD/KO Validated

ATP1B3 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1H9C1

Kat-Nr. : 67554-1-Ig

Synonyme

ATP1B3, ATPB 3, CD298, FLJ29027

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 67554-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 67554-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67554-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67554-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67554-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67554-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67554-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67554-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67554-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IHC: See 1 publications below

Produktinformation

67554-1-Ig bindet in WB, IHC, ELISA ATP1B3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATP1B3 fusion protein Ag30127
• Vollständiger Name: ATPase, Na+/K+ transporting, beta 3 polypeptide
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 38-43 kDa
• GenBank-Zugangsnummer: BC011835
• Gene symbol: ATP1B3
• Gene ID (NCBI): 483
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).

Publikationen

Species	Application	Title
human	IHC	Front Immunol; Integrative Transcriptomic, Proteomic and Functional Analysis Reveals ATP1B3 as a Diagnostic and Potential Therapeutic Target in Hepatocellular Carcinoma.; Authors - Shanshan Lu ; KD Validated