ATM Polyklonaler Antikörper

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• KD/KO Validated

ATM Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

29

Kat-Nr. : 27156-1-AP

Synonyme

A T mutated, AT1, ATA, ataxia telangiectasia mutated, ATC, ATD, ATDC, ATE, ATM, Serine protein kinase ATM, TEL1, TELO1

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Galerie der Validierungsdaten

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  WB analysis using 27156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27156-1-AP (ATM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27156-1-AP

  WB result of ATM antibody (27156-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATM transfected HEK-293 cells.

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  IP experiment of HEK-293 using 27156-1-AP

  IP result of anti-ATM(IP:27156-1-AP, 4ug; Detection:27156-1-AP 1:800) with HEK-293 cells lysate 1480 ug.

• 

  IHC staining of human breast cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 27156-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27156-1-AP (ATM antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 28 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

27156-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA ATM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATM fusion protein Ag25909
• Vollständiger Name: ataxia telangiectasia mutated
• Beobachtetes Molekulargewicht: 310-350 kDa
• GenBank-Zugangsnummer: BC137169
• Gene symbol: ATM
• Gene ID (NCBI): 472
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.

Publikationen

Species	Application	Title
human	WB	Sci Transl Med; Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer; Authors - Jia-Han Ding
human	WB	Cell Death Differ; p53-dependent elimination of aneuploid mitotic offspring by entosis.; Authors - Jianqing Liang
human	WB	Oncogene; TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex; Authors - Ya Wang
	WB	Clin Transl Med; Single-cell profiling-guided combination therapy of c-Fos and histone deacetylase inhibitors in diffuse large B-cell lymphoma.; Authors - Luqiao Wang
human	WB	EBioMedicine; MAP4K1 functions as a tumor promotor and drug mediator for AML via modulation of DNA damage/repair system and MAPK pathway.; Authors - Qing Ling
human	WB	Oncotarget; 2-Acetylamino-3-[4-(2-acetylamino-2-carboxyethylsulfanylcarbonylamino) phenyl carbamoylsulfanyl] propionic acid, a glutathione reductase inhibitor, induces G2/M cell cycle arrest through generation of thiol oxidative stress in human esophageal cancer cells.; Authors - Xia Li