ATG9A Polyklonaler Antikörper

ATG9A Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (2)

Kat-Nr. : 26276-1-AP

Synonyme

APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208

---

Galerie der Validierungsdaten

• 

  WB analysis using 26276-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26276-1-AP (ATG9A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 26276-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26276-1-AP (ATG9A Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 26276-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26276-1-AP (ATG9A Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 26276-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26276-1-AP (ATG9A Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 26276-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26276-1-AP (ATG9A Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse brain using 26276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 26276-1-AP (ATG9A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 26276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 26276-1-AP (ATG9A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

26276-1-AP bindet in WB, IHC, IF, ELISA ATG9A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATG9A fusion protein Ag24212
• Vollständiger Name: ATG9 autophagy related 9 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 837 aa, 94 kDa
• Beobachtetes Molekulargewicht: 94 kDa
• GenBank-Zugangsnummer: BC065534
• Gene symbol: ATG9A
• Gene ID (NCBI): 79065
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.(PMID: 29437695, 29568063)

Publikationen

Species	Application	Title
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
rat	WB	Acta Pharm Sin B; MicroRNA-34c-5p provokes isoprenaline-induced cardiac hypertrophy by modulating autophagy via targeting ATG4B.; Authors - Yuhong Zhang
mouse	WB	Front Immunol; MicroRNA-34a Inhibition Alleviates Lung Injury in Cecal Ligation and Puncture Induced Septic Mice.; Authors - Song Chen
human	WB	DNA Cell Biol; Long Noncoding RNA KIF9-AS1 Regulates Transforming Growth Factor-β and Autophagy Signaling to Enhance Renal Cell Carcinoma Chemoresistance via microRNA-497-5p.; Authors - Yichen Jin
mouse	WB	Mol Neurobiol; RAB7A GTPase Is Involved in Mitophagosome Formation and Autophagosome-Lysosome Fusion in N2a Cells Treated with the Prion Protein Fragment 106-126; Authors - Zhiping Li
		Cell Biol Int; TFAM knockdown undermines SQSTM1 mRNA stability but retards autophagy flux and inhibits tumor cells proliferation under starvation conditions; Authors - Xu Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon