ATG4B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATG4B Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 15131-1-AP

Synonyme

APG4B, ATG4B, AUTL1, Autophagin 1, Cysteine protease ATG4B, hAPG4B, KIAA0943

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Galerie der Validierungsdaten

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  WB analysis using 15131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15131-1-AP (ATG4B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15131-1-AP

  WB result of ATG4B antibody (15131-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG4B transfected HEK-293 cells.

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  WB analysis using 15131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15131-1-AP (ATG4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15131-1-AP (ATG4B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15131-1-AP

  IP result of anti-ATG4B(IP:15131-1-AP, 4ug; Detection:15131-1-AP 1:800) with HeLa cells lysate 1560 ug.

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  IHC staining of human pancreas cancer using 15131-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15131-1-AP (ATG4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15131-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15131-1-AP (ATG4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15131-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15131-1-AP (ATG4B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse heart using 15131-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15131-1-AP (ATG4B antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

15131-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ATG4B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATG4B fusion protein Ag7261
• Vollständiger Name: ATG4 autophagy related 4 homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC000719
• Gene symbol: ATG4B
• Gene ID (NCBI): 23192
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG4B(autophagy 4) is also named as APG4B, AUTL1, KIAA0943 and belongs to the peptidase C54 family. It is a homolog of yeast Apg4, a cysteine protease involved in autophagy and is a cytoplasmic enzyme. This protein is highly expressed in skeletal muscle, with lower expression in heart, liver, and pancreas and no expression is detected in fetal tissues by the northern blot. ATG4B is widely expressed in tumor cell lines (PMID:12446702). It has some isoforms produced by alternative splicing with molecular mass of 31-52 kDa.

Publikationen

Species	Application	Title
human	WB,IF,IP	Autophagy; ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1; Authors - Weihong Xie ; KO Validated
rat	WB	Autophagy; RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.; Authors - Luis Muniz-Feliciano
human	WB,IF	Free Radic Biol Med; The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells.; Authors - Zhiguo Li ; KD Validated
human	WB	Cell Biosci; Discovery and mechanism studies of a novel ATG4B inhibitor Ebselen by drug repurposing and its anti-colorectal cancer effects in mice; Authors - Huazhong Xie ; KO Validated
human	WB	Cancer Lett; HSF1 upregulates ATG4B expression and enhances epirubicin-induced protective autophagy in hepatocellular carcinoma cells.; Authors - Nan Zhang
human	WB	Front Cell Dev Biol; LncRNA CRNDE Promotes ATG4B-Mediated Autophagy and Alleviates the Sensitivity of Sorafenib in Hepatocellular Carcinoma Cells.; Authors - Lingxi Chen ; KD Validated