ATG16L1 Polyklonaler Antikörper

ATG16L1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (5)

Kat-Nr. : 19812-1-AP

Synonyme

APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, IBD10, WDR30

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse spleen using 19812-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19812-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19812-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19812-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 19812-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 19812-1-AP

  IP Result of anti-ATG16L1 (IP:19812-1-AP, 4ug; Detection:19812-1-AP 1:300) with MCF-7 cells lysate 2000ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, HEK-293T-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

19812-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ATG16L1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATG16L1 fusion protein Ag13844
• Vollständiger Name: ATG16 autophagy related 16-like 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 607 aa, 68 kDa
• Beobachtetes Molekulargewicht: 63-71 kDa
• GenBank-Zugangsnummer: BC000061
• Gene symbol: ATG16L1
• Gene ID (NCBI): 55054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)

Publikationen

Species	Application	Title
human	IF	Autophagy; ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1; Authors - Weihong Xie
human	IF	Nat Commun; Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates.; Authors - Shuang-Zhou Peng
human	WB	Autophagy; AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.; Authors - Ashwinkumar Subramenium Ganapathy
human	WB	Curr Biol; Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.; Authors - Anna Ulbricht
human	WB,IHC	Cancer Lett; Let-7i-5p promotes a malignant phenotype in nasopharyngeal carcinoma via inhibiting tumor-suppressive autophagy.; Authors - Bo You
human	WB	Clin Transl Immunology; Circular RNA TRAPPC6B inhibits intracellular Mycobacterium tuberculosis growth while inducing autophagy in macrophages by targeting microRNA-874-3p.; Authors - Hou-Long Luo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Priya (Verified Customer) (07-31-2023)

Used for Caco2 cells and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 2000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 2000
• Cell Tissue Type: Caco2, Mice colon

FH

X (Verified Customer) (01-18-2021)

It is OK to use it in WB with human postmortem brain lysate. There is band with MW as expected, although there is other unidentified bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human postmortem brain lysate

ATG16L1 Antibody Western Blot validation (1:1000 dilution) in human postmortem brain lysate (Cat no:19812-1-AP)