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- KD/KO Validated
ATF6 Monoklonaler Antikörper
ATF6 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Maus, Ratte und mehr (1)
Anwendung
WB, IHC, IF, FC, ELISA
Konjugation
Unkonjugiert
CloneNo.
3B7E4
Kat-Nr. : 66563-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | U2OS-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen |
Erfolgreiche Detektion in IHC | humanes Zervixkarzinomgewebe, humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF | HepG2-Zellen |
Erfolgreiche Detektion in FC | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunhistochemie (IHC) | IHC : 1:250-1:1000 |
Immunfluoreszenz (IF) | IF : 1:50-1:500 |
Durchflusszytometrie (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 20 publications below |
IF | See 2 publications below |
Produktinformation
66563-1-Ig bindet in WB, IHC, IF, FC, ELISA ATF6 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | ATF6 fusion protein Ag21456 |
Vollständiger Name | activating transcription factor 6 |
Berechnetes Molekulargewicht | 75 kDa |
Beobachtetes Molekulargewicht | 90-100 kDa |
GenBank-Zugangsnummer | BC014969 |
Gene symbol | ATF6 |
Gene ID (NCBI) | 22926 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for ATF6 antibody 66563-1-Ig | Protokoll herunterladen |
IHC protocol for ATF6 antibody 66563-1-Ig | Protokoll herunterladen |
IF protocol for ATF6 antibody 66563-1-Ig | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Protein Cell POST1/C12ORF49 regulates the SREBP pathway by promoting site-1 protease maturation. | ||
J Cell Biol Antigen-derived peptides engage the ER stress sensor IRE1α to curb dendritic cell cross-presentation. | ||
Cell Rep CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance | ||
Elife Endoplasmic reticulum stress activates human IRE1α through reversible assembly of inactive dimers into small oligomers. | ||
Mol Oncol The synthetic oleanane triterpenoid CDDO-2P-Im binds GRP78/BiP to induce unfolded protein response-mediated apoptosis in myeloma | ||
J Inflamm (Lond) Soluble epoxide hydrolase deficiency attenuates airway inflammation in COPD via IRE1α/JNK/AP-1 signaling pathway |