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ATF6 Monoklonaler Antikörper

ATF6 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3B7E4

Kat-Nr. : 66563-1-Ig

Synonyme

ATF6, ATF6 alpha, ATF6A



Geprüfte Anwendungen

Erfolgreiche Detektion in WBU2OS-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
Erfolgreiche Detektion in IHChumanes Zervixkarzinomgewebe, humanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:250-1:1000
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66563-1-Ig bindet in WB, IHC, IF, FC, ELISA ATF6 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen ATF6 fusion protein Ag21456
Vollständiger Name activating transcription factor 6
Berechnetes Molekulargewicht 75 kDa
Beobachtetes Molekulargewicht 90-100 kDa
GenBank-ZugangsnummerBC014969
Gene symbol ATF6
Gene ID (NCBI) 22926
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.

Protokolle

Produktspezifische Protokolle
WB protocol for ATF6 antibody 66563-1-IgProtokoll herunterladen
IHC protocol for ATF6 antibody 66563-1-IgProtokoll herunterladen
IF protocol for ATF6 antibody 66563-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Protein Cell

POST1/C12ORF49 regulates the SREBP pathway by promoting site-1 protease maturation.

Authors - Jian Xiao
mouseWB

J Cell Biol

Antigen-derived peptides engage the ER stress sensor IRE1α to curb dendritic cell cross-presentation.

Authors - Ofer Guttman
humanWB

Cell Rep

CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance

Authors - Masaki Takasugi
humanWB

Elife

Endoplasmic reticulum stress activates human IRE1α through reversible assembly of inactive dimers into small oligomers.

Authors - Vladislav Belyy
humanWB

Mol Oncol

The synthetic oleanane triterpenoid CDDO-2P-Im binds GRP78/BiP to induce unfolded protein response-mediated apoptosis in myeloma

Authors - George Luo
humanWB,IF

J Inflamm (Lond)

Soluble epoxide hydrolase deficiency attenuates airway inflammation in COPD via IRE1α/JNK/AP-1 signaling pathway

Authors - Yue Yu