ATF4 Monoklonaler Antikörper
ATF4 Monoklonal Antikörper für FC (Intra), IF
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Maus
Anwendung
IF, FC (Intra)
Konjugation
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3E4C5
Kat-Nr. : CL488-60035
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in IF | HeLa-Zellen |
Erfolgreiche Detektion in FC | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Immunfluoreszenz (IF) | IF : 1:50-1:500 |
Durchflusszytometrie (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
CL488-60035 bindet in IF, FC (Intra) ATF4 und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | ATF4 fusion protein Ag1279 |
Vollständiger Name | activating transcription factor 4 (tax-responsive enhancer element B67) |
Berechnetes Molekulargewicht | 39 kDa |
Beobachtetes Molekulargewicht | 45-50 kDa |
GenBank-Zugangsnummer | BC022088 |
Gene symbol | ATF4 |
Gene ID (NCBI) | 468 |
Konjugation | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3. |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. This antibody is a mouse monoclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38 kd ATF4 protein and its phosphorylated forms (50 kd). This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
Protokolle
Produktspezifische Protokolle | |
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IF protocol for CL Plus 488 ATF4 antibody CL488-60035 | Protokoll herunterladen |
FC protocol for CL Plus 488 ATF4 antibody CL488-60035 | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |