ASPH Polyklonaler Antikörper

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• KD/KO Validated

ASPH Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14116-1-AP

Synonyme

AAH, ASP beta hydroxylase, aspartate beta hydroxylase, ASPH, BAH, CASQ2BP1, HAAH, JCTN, junctin

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Galerie der Validierungsdaten

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  WB analysis of A549 using 14116-1-AP

  WB result of ASPH antibody (14116-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASPH transfected A549 cells.

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  WB analysis of mouse brain using 14116-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14116-1-AP (ASPH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14116-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14116-1-AP (ASPH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14116-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14116-1-AP (ASPH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14116-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14116-1-AP (ASPH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14116-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14116-1-AP (ASPH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

14116-1-AP bindet in WB, IHC, ELISA ASPH und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASPH fusion protein Ag5265
• Vollständiger Name: aspartate beta-hydroxylase
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC066929
• Gene symbol: ASPH
• Gene ID (NCBI): 444
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aspartate-β-hydroxylase (ASPH; termed AAH in older literature) has been implicated in the cross-talk among all of these signaling pathways. Correspondingly, ASPH is expressed at high levels in many malignant neoplasms of different histogeneses. It has the 86-141 kDa bands (native and phosphorylated forms), cleavage products of 35-56 and 22-26 kDa.(PMID:27981247/PMID: 10956665)

Publikationen

Species	Application	Title
human	WB	EBioMedicine; Hydroxylase Activity of ASPH Promotes Hepatocellular Carcinoma Metastasis Through Epithelial-to-Mesenchymal Transition Pathway.; Authors - Qifei Zou ; KD Validated
human	WB	J Immunol Res; Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma.; Authors - Yujiao Zhou
human,rat	WB,IHC	Heliyon; Labyrinthin: A distinct pan-adenocarcinoma diagnostic and immunotherapeutic tumor specific antigen.; Authors - Michael Babich
human	WB	Biochem Pharmacol; Inhibition of circular RNA ASPH reduces the proliferation and promotes the apoptosis of hepatic stellate cells in hepatic fibrosis; Authors - Hongwu Meng