ASMTL Polyklonaler Antikörper

ASMTL Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14981-1-AP

Synonyme

ASMTL, ASMTLX, ASMTLY, ASTML

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Galerie der Validierungsdaten

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  WB analysis of human testis using 14981-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14981-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14981-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 14981-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14981-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14981-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 14981-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14981-1-AP (ASMTL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 14981-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14981-1-AP (ASMTL antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A549 using 14981-1-AP

  Immunofluorescent analysis of A549 cells using 14981-1-AP (ASMTL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, A549-Zellen, HeLa-Zellen, HL-60-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14981-1-AP bindet in WB, IHC, IF,ELISA ASMTL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASMTL fusion protein Ag6894
• Vollständiger Name: acetylserotonin O-methyltransferase-like
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 69-75 kDa
• GenBank-Zugangsnummer: BC002508
• Gene symbol: ASMTL
• Gene ID (NCBI): 8623
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.