ASL Monoklonaler Antikörper

ASL Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1G9H3

Kat-Nr. : 67692-1-Ig

Synonyme

argininosuccinate lyase, Arginosuccinase, ASAL, ASL

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Galerie der Validierungsdaten

• 

  WB analysis using 67692-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67692-1-Ig (ASL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67692-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67692-1-Ig (ASL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67692-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67692-1-Ig (ASL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Nierengewebe, Hausschwein-Lebergewebe, K-562-Zellen, LNCaP-Zellen, Mauslebergewebe, Mausnierengewebe, Rattenlebergewebe, Rattennierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67692-1-Ig bindet in WB, ELISA ASL und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ASL fusion protein Ag30517
• Vollständiger Name: argininosuccinate lyase
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC008195
• Gene symbol: ASL
• Gene ID (NCBI): 435
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Argininosuccinate lyase (ASL), one of the significant UC enzymes, catalyzes argininosuccinate cleavage to generate arginine and fumarate. Arginine is then catalyzed by arginase to ornithine and polyamines, which are found to promote cancer cell proliferation and growth. Importantly, ASL ectopic expression is closely associated with poor prognosis of colorectal cancer, hepatocellular. ASL is a member of the aspartase/fumarase superfamily. Enzymes of this superfamily share similar tetrameric structure and active site, though the sequence identities between different members are quite low (less than 20%). Members of this superfamily have been recognised as drug targets for microbial infections. ASL is a 464-amino-acid enzyme with a molecular mass of 49.7 kDa. (PMID: 22386318 PMID: 31018905)

Publikationen

Species	Application	Title
mouse	WB	EMBO Mol Med; A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy; Authors - Nneka Southwell