ASCC3 Polyklonaler Antikörper

ASCC3 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 17627-1-AP

Synonyme

ASC 1 complex subunit p200, ASC1p200, ASCC3, HELIC1, Helicase, ATP binding 1, RNAH, Trip4 complex subunit p200

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 17627-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17627-1-AP (ASCC3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17627-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17627-1-AP (ASCC3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17627-1-AP

  IP Result of anti-ASCC3 (IP:17627-1-AP, 4ug; Detection:17627-1-AP 1:400) with HeLa cells lysate 1080ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IP: See 1 publications below

Produktinformation

17627-1-AP bindet in WB, IP, ELISA ASCC3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASCC3 fusion protein Ag11848
• Vollständiger Name: activating signal cointegrator 1 complex subunit 3
• Berechnetes Molekulargewicht: 111 aa, 13 kDa, 251 kDa
• Beobachtetes Molekulargewicht: 251 kDa
• GenBank-Zugangsnummer: BC050681
• Gene symbol: ASCC3
• Gene ID (NCBI): 10973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IP,WB	Nat Commun; Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex; Authors - Junqiao Jia
human	WB	Mol Cell; Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress.; Authors - Mark Stoneley
human	WB	Sci Rep; Identification of a novel trigger complex that facilitates ribosome-associated quality control in mammalian cells.; Authors - Satoshi Hashimoto
mouse	WB	Cell Metab; Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response; Authors - Goda Snieckute

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (10-24-2023)

(Already in contact with technical support, but was asked to submit a review here as well.) Immunoblotting for ASCC3 from fresh cell extracts did not work. Included samples of ASCC3-KD (siRNA) experiment, but only unspecific bands. Tested 1:1000 and 1:500 dilution. Tested different protein extraction buffers and conditions, used fresh cells each time. Transfer of large proteins was confirmed. Sadly, for us this antibody did not work.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 and 1:500
• Cell Tissue Type: HEK293