Beta Arrestin 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Beta Arrestin 1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 15361-1-AP

Synonyme

ARB1, ARR1, ARRB1, Arrestin beta 1, arrestin, beta 1, Beta arrestin 1, β-Arrestin-1

---

Galerie der Validierungsdaten

• 

  WB analysis using 15361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15361-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15361-1-AP (ARRB1 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 15361-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 15361-1-AP (ARRB1 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 15361-1-AP

  IP Result of anti-beta Arrestin 1 (IP:15361-1-AP, 4ug; Detection:15361-1-AP 1:500) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of human tonsillitis using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15361-1-AP (beta Arrestin 1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15361-1-AP (beta Arrestin 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, Mauslungengewebe, Rattenlungengewebe, RAW 264.7-Zellen, U-937-Zellen
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, Maus-Cerebellum-Gewebe, Maushirngewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

15361-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA Beta Arrestin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Beta Arrestin 1 fusion protein Ag7608
• Vollständiger Name: arrestin, beta 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47-55 kDa
• GenBank-Zugangsnummer: BC003636
• Gene symbol: ARRB1
• Gene ID (NCBI): 408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).

Publikationen

Species	Application	Title
human,mouse	WB,CoIP	Cell Metab; Autonomous sensing of the insulin peptide by an olfactory G protein-coupled receptor modulates glucose metabolism.; Authors - Jie Cheng ; KD Validated
mouse	IF	J Cell Biol; BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.; Authors - Fan Ye
	IF	Cell Death Dis; β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy.; Authors - J Liu
human	WB	J Biol Chem; An ionic lock and a hydrophobic zipper mediate the coupling between an insect pheromone receptor BmOR3 and downstream effectors.; Authors - Jing-Yu Lin ; KD Validated
human	WB	PLoS One; Cell Survival Following Radiation Exposure Requires miR-525-3p Mediated Suppression of ARRB1 and TXN1.; Authors - Kraemer Anne A
mouse	IF	Oncol Rep; Blue light irradiation inhibits the growth of colon cancer and activation of cancer‑associated fibroblasts.; Authors - Toshiaki Yoshimoto