ARPC5L Polyklonaler Antikörper

ARPC5L Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 22025-1-AP

Synonyme

ARC16 2, ARPC5L

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Galerie der Validierungsdaten

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  WB analysis using 22025-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 22025-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 22025-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 22025-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22025-1-AP

  IP Result of anti-ARPC5L (IP:22025-1-AP, 4ug; Detection:22025-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human placenta using 22025-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 22025-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cerebellum using 22025-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cerebellum using 22025-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 22025-1-AP

  Immunofluorescent analysis of MCF-7 cells using 22025-1-AP (ARPC5L antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of MCF-7 using 22025-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug ARPC5L antibody (22025-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Mauslungengewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

22025-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ARPC5L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARPC5L fusion protein Ag16881
• Vollständiger Name: actin related protein 2/3 complex, subunit 5-like
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17-20 kDa
• GenBank-Zugangsnummer: BC000798
• Gene symbol: ARPC5L
• Gene ID (NCBI): 81873
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARPC5L, also known as ARC16-2, belongs to the ARPC5 family. ARPC5L is involved in Arp2/3 complex-mediated actin nucleation and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. ARPC5L-containing Arp2/3 complexes are required for the peripheral positioning of nuclei in myofibers (PMID: 28892082). The molecular mass of ARPC5L is 17-20 kDa.

Publikationen

Species	Application	Title
human	WB,IF	Pigment Cell Melanoma Res; YAP facilitates melanoma migration through regulation of actin-related protein 2/3 complex subunit 5 (ARPC5); Authors - Jason W. Lui