ARPC3 Polyklonaler Antikörper

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• KD/KO Validated

ARPC3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 14652-1-AP

Synonyme

ARC21, Arp2/3 complex 21 kDa subunit, ARPC3

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14652-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14652-1-AP (ARPC3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 14652-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14652-1-AP (ARPC3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14652-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14652-1-AP (ARPC3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 14652-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14652-1-AP (ARPC3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 14652-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14652-1-AP (ARPC3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Produktinformation

14652-1-AP bindet in WB, IP, IHC, ELISA ARPC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARPC3 fusion protein Ag6249
• Vollständiger Name: actin related protein 2/3 complex, subunit 3, 21kDa
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC067747
• Gene symbol: ARPC3
• Gene ID (NCBI): 10094
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nat Immunol; Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.; Authors - Rui Martins
human	WB	J Cell Biol; α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3.; Authors - Nikki R Paul ; KD Validated
human	IP	EMBO Rep; WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis.; Authors - Kailiang Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kiara (Verified Customer) (11-15-2021)

Works great for Western Blot! We used 5% milk in TBS-T to block the membrane and diluted the ARPC3 antibody 1:1000 in 5% milk in TBS-T. We received one specific clear band. Awesome antibody!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neonatal microglia