ARPC2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ARPC2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 15058-1-AP

Synonyme

ARC34, Arp2/3 complex 34 kDa subunit, ARPC2, p34 Arc, PNAS 139, PRO2446

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Galerie der Validierungsdaten

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  WB analysis using 15058-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15058-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 15058-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15058-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15058-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15058-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15058-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15058-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15058-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15058-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15058-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15058-1-AP

  IP result of anti-ARPC2(IP:15058-1-AP, 4ug; Detection:15058-1-AP 1:6000) with HeLa cells lysate 1200 ug.

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  IHC staining of human breast cancer using 15058-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15058-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15058-1-AP

  Immunofluorescent analysis of Hela cells, using ARPC2 antibody 15058-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 15058-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARPC2 antibody (15058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 15058-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (15058-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, 3T3-L1-Zellen, HeLa-Zellen, Jurkat-Zellen, Maus-Skelettmuskelgewebe, MCF-7-Zellen, NIH/3T3-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

15058-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ARPC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARPC2 fusion protein Ag7041
• Vollständiger Name: actin related protein 2/3 complex, subunit 2, 34kDa
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC000590
• Gene symbol: ARPC2
• Gene ID (NCBI): 10109
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	WB	Genes Dev; Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein; Authors - Megan L Norris
human	WB	J Cell Biol; α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3.; Authors - Nikki R Paul ; KD Validated
human	IP	EMBO Rep; WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis.; Authors - Kailiang Zhao
human	WB	J Neurosci; The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse.; Authors - Avanti Gokhale
human	WB, IF	Front Cell Dev Biol; Feedback-Driven Mechanisms Between Phosphorylated Caveolin-1 and Contractile Actin Assemblies Instruct Persistent Cell Migration.; Authors - Xuemeng Shi
human	WB	Cell Rep; Substrate rigidity dictates colorectal tumorigenic cell stemness and metastasis via CRAD-dependent mechanotransduction.; Authors - Yuhan Chang