ARMC10 Polyklonaler Antikörper

ARMC10 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20506-1-AP

Synonyme

armadillo repeat containing 10, ARMC10, PNAS 112, SVH

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Galerie der Validierungsdaten

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  WB analysis of A431 using 20506-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20506-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20506-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20506-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20506-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 20506-1-AP

  IP Result of anti-ARMC10 (IP:20506-1-AP, 4ug; Detection:20506-1-AP 1:500) with A431 cells lysate 1600ug.

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  IHC staining of human placenta using 20506-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 20506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 20506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HepG2-Zellen, Maushirngewebe, Mauslebergewebe
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Produktinformation

20506-1-AP bindet in WB, IP, IHC, IF, ELISA ARMC10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARMC10 fusion protein Ag14313
• Vollständiger Name: armadillo repeat containing 10
• Berechnetes Molekulargewicht: 343 aa, 38 kDa
• Beobachtetes Molekulargewicht: 31-37 kDa
• GenBank-Zugangsnummer: BC003586
• Gene symbol: ARMC10
• Gene ID (NCBI): 83787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IP	Cell Death Dis; The Armc10/SVH gene: genome context, regulation of mitochondrial dynamics and protection against Aβ-induced mitochondrial fragmentation.; Authors - R Serrat
human	WB	Heliyon; Prognostic value and immune infiltration of ARMC10 in pancreatic adenocarcinoma via integrated bioinformatics analyses; Authors - Tian-Hao Li ; KD Validated