ARL13B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ARL13B Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-17711

Synonyme

ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8

---

Galerie der Validierungsdaten

• 

  IF Staining of MDCK using CL594-17711

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL594-17711 (ARL13B antibody) at dilution of 1:50. Cells were co-stained with CL488-66200 (acetylated Tubulin(Lys40) antibody).

• 

  IF Staining of MDCK using CL594-17711

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL594-17711 (ARL13B antibody) at dilution of 1:50 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of hTERT-RPE1 using CL594-17711

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite®594 ARL13B antibody (CL594-17711) at dilution of 1:200, acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, green).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: hTERT-RPE1-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-17711 bindet in IF ARL13B und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARL13B fusion protein Ag12015
• Vollständiger Name: ADP-ribosylation factor-like 13B
• Berechnetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC094725
• Gene symbol: ARL13B
• Gene ID (NCBI): 200894
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). CoraLite594-Conjugated ARL13B antibody can be used to mark the cilia(red). Ex: 593nm, Em: 614nm