ARL13B Monoklonaler Antikörper

ARL13B Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Hund, Maus und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

CloneNo.

1H6C3

Rezensionen (3)

Kat-Nr. : 66739-1-Ig

Synonyme

ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 66739-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66739-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66739-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse heart using 66739-1-Ig

  Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (magenta, Cat. No 15513-1-AP), CD31/PECAM-1 (white), and Arl13B (yellow, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (cyan). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to magenta.CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody and psedocolored to yellow. The Image and figure legends are intellectual property of @LAF_in_the_LAB.

• 

  IF Staining of MDCK using 66739-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 66739-1-Ig (ARL13B antibody) at dilution of 1:60 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of hTERT-RPE1 using 66739-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using ARL13B antibody (66739-1-Ig, Clone: 1H6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red).

• 

  IF Staining of mouse heart using 66739-1-Ig

  Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T /cTnT (red, Cat. No 15513-1-AP), CD31/PECAM-1 (magenta), and Arl13B (green, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IF: MDCK-Zellen, hTERT-RPE1-Zellen, Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 13 publications below

Produktinformation

66739-1-Ig bindet in WB, IF, ELISA ARL13B und zeigt Reaktivität mit Hausschwein, human, Hund, Maus

• Getestete Reaktivität: Hausschwein, human, Hund, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ARL13B fusion protein Ag12031
• Vollständiger Name: ADP-ribosylation factor-like 13B
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC094725
• Gene symbol: ARL13B
• Gene ID (NCBI): 200894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Publikationen

Species	Application	Title
human	IF,WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda
human	WB	Elife; Multiple ciliary localization signals control INPP5E ciliary targeting; Authors - Dario Cilleros-Rodriguez
human	IF	Sci Rep; Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts.; Authors - Elisa Molinari
rat	IF	eNeuro; CCP1, a Tubulin Deglutamylase, Increases Survival of Rodent Spinal Cord Neurons following Glutamate-Induced Excitotoxicity.; Authors - Yasmin H Ramadan
human	IF	PLoS One; Embryonic and foetal expression patterns of the ciliopathy gene CEP164.; Authors - L A Devlin
mouse	IF	Life Sci Alliance; HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails.; Authors - Pablo Barbeito

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lindsey (Verified Customer) (05-24-2021)

I much prefer the Rabbit polyclonal ARL13B (Cat#17711-I-AP) but it is really nice to have an Arl13B Mouse monoclonal for use in co-stains with other targets. I wish it didn't have to be used at such a high working concentration!

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human kidney (FFPE)

ARL13B Antibody Immunohistochemistry,Immunofluorescence validation (1:50 dilution) in Human kidney (FFPE) (Cat no:66739-1-Ig)

FH

Feng-Qian (Verified Customer) (04-04-2019)

This antibody works for detecting primary cilia in MEFs with low background. The intensity is lower than the Arl13B rabbit antibody (17711-1-AP) in general but specific.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: methanol fixed Mouse embryonic fibroblasts

FH

Francesc (Verified Customer) (11-07-2018)

This antibody works beautifully, staining cilia with high intensity and specificity, as expected for ARL13B.The staining with 66739-1-Ig appears as good as that obtained with the highly validated Proteintech's rabbit anti-ARL13B (17711-1-AP).This mouse anti-ARL13B makes for a great ciliary marker.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: IMCD3 cells (mouse kidney cell line)

ARL13B Antibody Immunofluorescence, validation (1:100 dilution) in IMCD3 cells (mouse kidney cell line) (Cat no:66739-1-Ig)