ARID1A Polyklonaler Antikörper

ARID1A Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30304-1-AP

Synonyme

ARID1A, B120, BAF250, BAF250a, BM029, BRG1 associated factor 250, BRG1 associated factor 250a, C1orf4, hELD, hOSA1, Osa homolog 1, OSA1, P270, SMARCF1, SWI like protein, SWI/SNF complex protein p270

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Galerie der Validierungsdaten

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  WB analysis using 30304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30304-1-AP (ARID1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 30304-1-AP

  IP result of anti-ARID1A(IP:30304-1-AP, 4ug; Detection:30304-1-AP 1:3000) with HeLa cells lysate 1280 ug.

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  IHC staining of human endometrial cancer using 30304-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 30304-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 30304-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 30304-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30304-1-AP bindet in WB, IP, IHC, ELISA ARID1A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARID1A fusion protein Ag30749
• Vollständiger Name: AT rich interactive domain 1A (SWI-like)
• Berechnetes Molekulargewicht: 242 kDa
• Beobachtetes Molekulargewicht: 250-260 kDa
• GenBank-Zugangsnummer: NM_006015
• Gene symbol: ARID1A
• Gene ID (NCBI): 8289
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARID1A, also named as BAF250, BAF250A, C1orf4, OSA1 and SMARCF1, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. ARID1A belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The antibody is specific to ARID1A.