ARHGDIB Polyklonaler Antikörper

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• KD/KO Validated

ARHGDIB Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 16122-1-AP

Synonyme

ARHGDIB, D4, GDIA2, GDID4, Ly GDI, LYGDI, RAP1GN1, Rho GDI 2, Rho GDI beta, RhoGDI2

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Galerie der Validierungsdaten

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  WB analysis using 16122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16122-1-AP (ARHGDIB antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 16122-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 16122-1-AP (ARHGDIB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 16122-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16122-1-AP (ARHGDIB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 16122-1-AP

  IP Result of anti-ARHGDIB (IP:16122-1-AP, 4ug; Detection:16122-1-AP 1:1000) with Raji cells lysate 2000ug.

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  IHC staining of human stomach cancer using 16122-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16122-1-AP (ARHGDIB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 16122-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16122-1-AP (ARHGDIB antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, Raji-Zellen, Rattenmilzgewebe
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

16122-1-AP bindet in WB, IP, IHC, ELISA ARHGDIB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARHGDIB fusion protein Ag9078
• Vollständiger Name: Rho GDP dissociation inhibitor (GDI) beta
• Berechnetes Molekulargewicht: 201 aa, 23 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC009200
• Gene symbol: ARHGDIB
• Gene ID (NCBI): 397
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.

Publikationen

Species	Application	Title
human	WB	Nat Metab; Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression; Authors - Lin Qian
human	WB	Nat Immunol; Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.; Authors - Jing Geng
human	WB	Mol Cancer; KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1.; Authors - Lei Liu
human	WB	Proteomics; Changes in the proteomic profile during the differential polarization status of the human monocyte-derived macrophage THP-1 cell line.; Authors - Fan Zhang
human	WB,IHC	Cancer Biomark; Systematic investigation of biomarker-like role of ARHGDIB in breast cancer.; Authors - Xiaonan Wang ; KD Validated
human	WB	DNA Cell Biol; Rho GDP Dissociation Inhibitor (GDI) Beta Promotes Cell Proliferation and Invasion by Modulating the AKT Pathway in Hepatocellular Carcinoma.; Authors - Yang Fang