GRAF Polyklonaler Antikörper

GRAF Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 17747-1-AP

Synonyme

ARHGAP26, GRAF, KIAA0621, Oligophrenin 1 like protein, OPHN1L, OPHN1L1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 17747-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17747-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17747-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17747-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17747-1-AP

  IP Result of anti-GRAF (IP:17747-1-AP, 4ug; Detection:17747-1-AP 1:1000) with HepG2 cells lysate 3600ug.

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  IHC staining of human liver using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Maus-Thymusgewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

17747-1-AP bindet in WB, IP, IHC, CoIP, ELISA GRAF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRAF fusion protein Ag12008
• Vollständiger Name: Rho GTPase activating protein 26
• Berechnetes Molekulargewicht: 759 aa, 86 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC068555
• Gene symbol: ARHGAP26
• Gene ID (NCBI): 23092
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	J Neurol; Rho GTPase-activating protein 10 (ARHGAP10/GRAF2) is a novel autoantibody target in patients with autoimmune encephalitis; Authors - Sven Jarius
human	WB	Brain Res; Deficiency of NEAT1 prevented MPP+-induced inflammatory response, oxidative stress and apoptosis in dopaminergic SK-N-SH neuroblastoma cells via miR-1277-5p/ARHGAP26 axis.; Authors - Shufang Zhou
human	WB,IHC,CoIP	Cell Death Dis; CEMIP, acting as a scaffold protein for bridging GRAF1 and MIB1, promotes colorectal cancer metastasis via activating CDC42/MAPK pathway; Authors - Guojie Xu