ARHGAP24 Polyklonaler Antikörper

ARHGAP24 Polyklonal Antikörper für IHC,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 18834-1-AP

Synonyme

ARHGAP24, Filamin A associated RhoGAP, FILGAP, p73, p73RhoGAP, RC GAP72, RCGAP72, Rho type GTPase activating protein 24, RhoGAP of 73 kDa, Sarcoma antigen NY SAR 88

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Galerie der Validierungsdaten

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  IHC staining of human kidney using 18834-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18834-1-AP (ARHGAP24 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18834-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18834-1-AP (ARHGAP24 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

18834-1-AP bindet in WB, IHC, IF, ELISA ARHGAP24 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Rho GTPase activating protein 24
• Berechnetes Molekulargewicht: 84 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC098580
• Gene symbol: ARHGAP24
• Gene ID (NCBI): 83478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARHGAP24, also named as FILGAP, is involved in cell polarity, cell morphology and cytoskeletal organization. ARHGAP24 acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. ARHGAP24 controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. ARHGAP24 can suppress the activity of RAC1 and CDC42 in vitro. Its overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis. The antibody may recognize all the isoforms of ARHGAP24 except isoform 5.

Publikationen

Species	Application	Title
human	IF	Curr Biol; Cyclic Mechanical Loading Is Essential for Rac1-Mediated Elongation and Remodeling of the Embryonic Mitral Valve.; Authors - Russell A Gould
human	WB,IHC	Oncogene; Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma.; Authors - Xin Jin
human	IHC	Oncotarget; Global gene expression in pseudomyxoma peritonei, with parallel development of two immortalized cell lines.; Authors - Darren L Roberts
human	WB	Clin Exp Pharmacol Physiol; ARHGAP17 inhibits pathological cyclic strain-induced apoptosis in human periodontal ligament fibroblasts via Rac1/Cdc42.; Authors - Li Wang
human,mouse	WB,IF	Biochem Biophys Rep; Knockdown of ARHGAP24 reduces intimal hyperplasia through inhibiting the proliferation and phenotypic switching of smooth muscle cells possibly by inactivating both AKT and ERK1/2 signaling pathways; Authors - Wei Liu