ARHGAP10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ARHGAP10 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 55139-1-AP

Synonyme

ARHGAP10, Graf related protein 2, GRAF2, PS GAP, PSGAP

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 55139-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 55139-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 55139-1-AP (ARHGAP10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 55139-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55139-1-AP (ARHGAP10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55139-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55139-1-AP (ARHGAP10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55139-1-AP (ARHGAP10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55139-1-AP (ARHGAP10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HEK-293-Zellen, humanes Herzgewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below

Produktinformation

55139-1-AP bindet in WB, IHC, ELISA ARHGAP10 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Rho GTPase activating protein 10
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 89 kDa
• GenBank-Zugangsnummer: NM_024605
• Gene symbol: ARHGAP10
• Gene ID (NCBI): 79658
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARHGAP10, also named as GRAF2, is GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. It is essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. ARHGAP10 inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. It stabilizes PAK-2p34 thereby increasing stimulation of cell death. The antibody is specific to ARHGAP10.

Publikationen

Species	Application	Title
human	WB	Nat Metab; Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression; Authors - Lin Qian
human	WB	Mol Ther Nucleic Acids; miR-3174 Contributes to Apoptosis and Autophagic Cell Death Defects in Gastric Cancer Cells by Targeting ARHGAP10.; Authors - Bowen Li ; KD Validated
mouse	WB	Front Cell Dev Biol; The Beta-Tubulin Isotype TUBB6 Controls Microtubule and Actin Dynamics in Osteoclasts.; Authors - Justine Maurin
human	IHC	Front Oncol; Identification and Validation of an Apoptosis-Related Gene Prognostic Signature for Oral Squamous Cell Carcinoma.; Authors - Shuqin Wang
mouse	WB	J Biol Chem; The Cdc42 Guanine Nucleotide Exchange Factor FGD6 Coordinates Cell Polarity and Endosomal Membrane Recycling in Osteoclasts.; Authors - Charlotte Steenblock
mouse	WB	J Neurol; Rho GTPase-activating protein 10 (ARHGAP10/GRAF2) is a novel autoantibody target in patients with autoimmune encephalitis; Authors - Sven Jarius